recombinant mouse klotho protein  (R&D Systems)

Journal: bioRxiv

Article Title: Chemotherapy accelerated bone ageing is reversed by NMN

doi: 10.1101/2025.10.13.679926

Figure Lengend Snippet: Mice were treated with CDDP and NMN as per , and serum collected at 10 weeks of age for measurements of the renal function markers A) blood urea nitrogen (BUN) and B) cystatin C. Renal damage that can impact the excretion of C) phosphate (PO 4 ), which was not altered by these treatments, in contrast to D) elemental phosphorus, which encompasses both soluble, free phosphate, insoluble calcium phosphate precipitates, and organic phosphorus, such as phospholipids. Phosphate homeostasis can be regulated by the bone-derived hormone E) FGF23 and its coreceptor F) Klotho. These also regulate the production of G) parathyroid hormone (PTH), which maintain H) serum calcium levels and promote bone loss through the mobilisation of calcium from bone mineral stores. N=5-6 per group as indicated by data points, p-values are from Bonferroni-adjusted t-tests derived from estimated marginal means of linear model of NMN and CDDP treatment, with results of linear models indicated on each panel

Article Snippet: ELISA kits were used to measure cystatin C (Invitrogen EMCST3), Klotho (Cusabio CSB-E14362m) and FGF23 (Abcam ab213863).

Techniques: Derivative Assay

Journal: bioRxiv

Article Title: sKL/mKL Transcript Ratio and Protein Localization Define a Species- and Region-Specific Klotho Signature in the CNS and AD Progression

doi: 10.1101/2025.05.13.653838

Figure Lengend Snippet: Representative images of double immunolabelling sections from human hippocampus and amygdala for total klotho and s-KL (A) ; or for s-KL and GFAP (B) . Negative controls were done by incubating sections without the primary antibodies or by incubating with the pre-adsorbed antibody. White arrows in (B) point to the few neurons expressing s-KL, while yellow labelling indicates s-KL positive astrocytes. Bar scale size is 10μm. Quantification of total soluble klotho (KL1) and s-KL in CSF (C) and homogenized brain tissue (HC: hippocampus, EC: entorhinal cortex) (D) by ELISA.

Article Snippet: The primary antibody, mouse monoclonal klotho antibody F-5 (Santa Cruz Biotechnology, ref sc-515939), diluted 1/100 in blocking buffer, was applied overnight at 4°C.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Animals : an Open Access Journal from MDPI

Article Title: Characterization of Urine-Derived Stromal/Stem Cells from Healthy Dogs and Dogs Affected by Chronic Kidney Disease (CKD)

doi: 10.3390/ani15020242

Figure Lengend Snippet: Klotho protein expression was confirmed by Western blotting. Results are shown as the mean ± SEM. * p < 0.05. Legend: hUSCs = USCs from healthy dogs; sUSCs: = USCs from sick dogs.

Article Snippet: The membrane was cut into two pieces: one was hybridized with 1 μg of mouse monoclonal primary antibody against Klotho protein (sc-515942 Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and the other with 1 μg of anti GAPDH (sc-47724 Santa Cruz Biotechnology, Inc., Dallas, TX, USA) by the SNAP i.d.

Techniques: Expressing, Western Blot

Journal: Scientific Reports

Article Title: Klotho enhances stability of chronic kidney disease atherosclerotic plaques by inhibiting GRK2/PLC-β-mediated endoplasmic reticulum stress in macrophages via modulation of the ROS/SHP1 pathway

doi: 10.1038/s41598-024-83596-w

Figure Lengend Snippet: Klotho can inhibit the progression of atherosclerosis in chronic renal failure. A Oil red O staining results; B Statistics on the proportion of positive staining of oil red O; C Plots of the results of the qPCR experiment in the sham group, klotho-NC group and klotho-mimic group. N = 8; ** P < 0.01.

Article Snippet: Lentiviral vectors carrying full-length mouse Klotho cDNA (LV-KL) labeled with green fluorescent protein (GFP) were obtained from OriGene Technologies and accompanied by a negative control.

Techniques: Staining

Journal: Scientific Reports

Article Title: Klotho enhances stability of chronic kidney disease atherosclerotic plaques by inhibiting GRK2/PLC-β-mediated endoplasmic reticulum stress in macrophages via modulation of the ROS/SHP1 pathway

doi: 10.1038/s41598-024-83596-w

Figure Lengend Snippet: Klotho reduces the content of macrophages in atherosclerotic plaques in chronic renal failure. A HE staining results and statistical analysis of atherosclerotic plaque area; B Immunohistochemical staining results for MAC-2 and statistical analysis of expression data; C Immunohistochemical staining results for α-SMA and statistical analysis of expression data. N = 8;** P < 0.01; ns P > 0.05.

Article Snippet: Lentiviral vectors carrying full-length mouse Klotho cDNA (LV-KL) labeled with green fluorescent protein (GFP) were obtained from OriGene Technologies and accompanied by a negative control.

Techniques: Staining, Immunohistochemical staining, Expressing

Journal: Scientific Reports

Article Title: Klotho enhances stability of chronic kidney disease atherosclerotic plaques by inhibiting GRK2/PLC-β-mediated endoplasmic reticulum stress in macrophages via modulation of the ROS/SHP1 pathway

doi: 10.1038/s41598-024-83596-w

Figure Lengend Snippet: Klotho reduces the concentrations of urea, cholesterol, calcium ions, and triglycerides in chronic renal failure with atherosclerosis. A ELISA detection of urea concentrations in mouse plasma; B ELISA detection of cholesterol concentrations in mouse plasma; C ELISA detection of calcium ion concentrations in mouse plasma; D ELISA detection of triglyceride concentrations in mouse plasma. E: Masson staining results were plotted and the area of positive MASSON staining (blue area) was counted. N = 8;** P < 0.01; * P < 0.05.

Article Snippet: Lentiviral vectors carrying full-length mouse Klotho cDNA (LV-KL) labeled with green fluorescent protein (GFP) were obtained from OriGene Technologies and accompanied by a negative control.

Techniques: Enzyme-linked Immunosorbent Assay, Staining

Journal: Scientific Reports

Article Title: Klotho enhances stability of chronic kidney disease atherosclerotic plaques by inhibiting GRK2/PLC-β-mediated endoplasmic reticulum stress in macrophages via modulation of the ROS/SHP1 pathway

doi: 10.1038/s41598-024-83596-w

Figure Lengend Snippet: Klotho enhances the stability of atherosclerotic plaques in chronic renal failure. A Protein bands and relative protein expression levels of NOX2, NOX4, Caspase-3, Caspase-9, Bax and p-PERK as well as statistical analysis; B Protein bands and relative protein expression levels of p-GRK2, p-PLCβ and p-IP3R, as well as statistical analysis. C Protein bands and relative protein expression levels of p-SHP1 and p-Src, as well as statistical analysis.GAPDH as the control protein. N = 8; ** P < 0.01.

Article Snippet: Lentiviral vectors carrying full-length mouse Klotho cDNA (LV-KL) labeled with green fluorescent protein (GFP) were obtained from OriGene Technologies and accompanied by a negative control.

Techniques: Expressing, Control

Journal: Scientific Reports

Article Title: Klotho enhances stability of chronic kidney disease atherosclerotic plaques by inhibiting GRK2/PLC-β-mediated endoplasmic reticulum stress in macrophages via modulation of the ROS/SHP1 pathway

doi: 10.1038/s41598-024-83596-w

Figure Lengend Snippet: Klotho enhances the stability of atherosclerotic plaques through ROS/SHP1 pathway inhibition, downregulating GRK2/PLC-β-mediated ER stress. A Protein bands and relative protein expression levels of Klotho, NOX2, p-SHP1, p-Src, p-PERK, p-GRK2, and p-PLCβ, as well as statistical analysis; B Protein bands and relative protein expression levels of NOX2, p-SHP1, p-GRK2, p-PLCβ, and p-PERK, as well as statistical analysis. GAPDH as the control protein. N = 3; ** P < 0.01; ns P > 0.05.

Article Snippet: Lentiviral vectors carrying full-length mouse Klotho cDNA (LV-KL) labeled with green fluorescent protein (GFP) were obtained from OriGene Technologies and accompanied by a negative control.

Techniques: Inhibition, Expressing, Control

Journal: Scientific Reports

Article Title: Klotho enhances stability of chronic kidney disease atherosclerotic plaques by inhibiting GRK2/PLC-β-mediated endoplasmic reticulum stress in macrophages via modulation of the ROS/SHP1 pathway

doi: 10.1038/s41598-024-83596-w

Figure Lengend Snippet: Klotho enhances the stability of atherosclerotic plaques in chronic renal failure combined with atherosclerosis by inhibiting the ROS/SHP1 pathway and downregulating GRK2/PLC-β-mediated ER stress in macrophages.

Article Snippet: Lentiviral vectors carrying full-length mouse Klotho cDNA (LV-KL) labeled with green fluorescent protein (GFP) were obtained from OriGene Technologies and accompanied by a negative control.

Techniques:

Journal: Kidney Research and Clinical Practice

Article Title: Pan-Nox inhibitor treatment improves renal function in aging murine diabetic kidneys

doi: 10.23876/j.krcp.23.004

Figure Lengend Snippet: (A) Representative immunoblots for plasminogen activator inhibitor 1 (PAI-1) and type IV collagen (Col4); (B) densitometric analysis of immunoblots for PAI-1 and Col4; (C) representative immunoblot for Klotho, p-FoxO1, t-FoxO1 and Nrf2; and (D) densitometric analysis of immunoblot for Klotho, p-FoxO1/t-FoxO1 ratio, Nrf2. Data are shown as the mean ± standard error of mean. * p < 0.05, p < 0.001 vs. control or APX-115, # p < 0.05, ### p < 0.001 vs. streptozotocin (STZ).

Article Snippet: Proteins were transferred onto a polyvinylidene difluoride membrane, and the membrane was hybridized in blocking buffer overnight at 4 °C with rabbit polyclonal anti-Nox1 antibody (1:1,000; Abcam Plc), rabbit polyclonal anti-Nox2 antibody (1:1,000; Bioworld Technology), rabbit polyclonal anti-Nox4 antibody (1:1,000; Bioworld Technology), rabbit polyclonal anti-PAI-1 antibody (1:1,000; Santa Cruz Biotechnology), mouse monoclonal anti-monocyte chemoattractant protein (MCP)-1 antibody (1:1000; Lsbio), rabbit polyclonal anti-type IV collagen antibody (1:1,000; Abcam Plc), rabbit polyclonal anti-TGF-β1 antibody (1:1,000; Abbkine), goat polyclonal anti-Klotho antibody (1:1,000; R&D Systems Inc.), rabbit polyclonal anti-phospho FoxO1 antibody (1:1,000; Lsbio), rabbit polyclonal anti-total-FoxO1 antibody (1:1,000; Cell Signaling Technology), rabbit polyclonal anti-NRF2 antibody (1:1,000; Cell Signaling Technology), or mouse monoclonal anti-β actin antibody (1:5,000; Sigma-Aldrich).

Techniques: Western Blot, Control